Patient propagules: Do soil archives preserve the legacy of fungal and prokaryotic communities?
Identifieur interne : 000225 ( Main/Exploration ); précédent : 000224; suivant : 000226Patient propagules: Do soil archives preserve the legacy of fungal and prokaryotic communities?
Auteurs : Gian Maria Niccol Benucci [États-Unis] ; Bryan Rennick [États-Unis] ; Gregory Bonito [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Champignons.
- métabolisme : Cellules procaryotes.
- classification : Analyse de séquence, Biodiversité, Champignons, Microbiologie du sol, Écosystème.
English descriptors
- KwdEn :
- MESH :
- classification : Fungi.
- genetics : Fungi.
- metabolism : Prokaryotic Cells.
- Biodiversity, Ecosystem, Sequence Analysis, Soil Microbiology.
Abstract
Soil archives are an important resource in agronomic and ecosystem sciences. If microbial communities could be reconstructed from archived soil DNA, as prehistoric plant communities are reconstructed via pollen data, soil archive resources would assume even greater value for reconstructing land-use history, forensic science, and biosphere modelling. Yet, the effects of long-term soil archival on the preservation of microbial DNA is still largely unknown. To address this, we assessed the capacity of high-throughput sequencing (Illumina MiSeq) of ITS (internal transcribed spacer) and prokaryotic 16S rRNA genes for reconstructing soil microbial communities across a 20 years time-series. We studied air-dried soil archives and fresh soil samples taken from Populus bioenergy and deciduous forest research plots at the Kellogg Biological Station. Habitat and archival time explained significant amounts of variation in soil microbial α- and β-diversity both in fungal and prokaryotic communities. We found that microbial richness, diversity, and abundance generally decreased with storage time, but varied between habitat and taxonomic groups. The high relative abundance of ectomycorrhizal species including Hebeloma and Cortinarius detected in older soil archives raises questions regarding traits such as long-term persistence and viability of ectomycorrhizal propagules in soils, with relevance to forest health and ecosystem succession. Talaromyces, Paecilomyces and Epicoccum spp. were detected in fresh and across 20-year-old archived soils and were also cultured from these soils demonstrating their long-term spore viability. In summary, we found that microbial DNA in air-dried soils archived over the past 20 years degraded with time, in a manner that differed between soil types and phylogenetic groups of microbes.
DOI: 10.1371/journal.pone.0237368
PubMed: 32780777
PubMed Central: PMC7418970
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(MeSH)</term><term>Cellules procaryotes (métabolisme)</term><term>Champignons (classification)</term><term>Champignons (génétique)</term><term>Microbiologie du sol (MeSH)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Prokaryotic Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules procaryotes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodiversity</term><term>Ecosystem</term><term>Sequence Analysis</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Analyse de 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If microbial communities could be reconstructed from archived soil DNA, as prehistoric plant communities are reconstructed via pollen data, soil archive resources would assume even greater value for reconstructing land-use history, forensic science, and biosphere modelling. Yet, the effects of long-term soil archival on the preservation of microbial DNA is still largely unknown. To address this, we assessed the capacity of high-throughput sequencing (Illumina MiSeq) of ITS (internal transcribed spacer) and prokaryotic 16S rRNA genes for reconstructing soil microbial communities across a 20 years time-series. We studied air-dried soil archives and fresh soil samples taken from Populus bioenergy and deciduous forest research plots at the Kellogg Biological Station. Habitat and archival time explained significant amounts of variation in soil microbial α- and β-diversity both in fungal and prokaryotic communities. We found that microbial richness, diversity, and abundance generally decreased with storage time, but varied between habitat and taxonomic groups. The high relative abundance of ectomycorrhizal species including Hebeloma and Cortinarius detected in older soil archives raises questions regarding traits such as long-term persistence and viability of ectomycorrhizal propagules in soils, with relevance to forest health and ecosystem succession. Talaromyces, Paecilomyces and Epicoccum spp. were detected in fresh and across 20-year-old archived soils and were also cultured from these soils demonstrating their long-term spore viability. In summary, we found that microbial DNA in air-dried soils archived over the past 20 years degraded with time, in a manner that differed between soil types and phylogenetic groups of microbes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32780777</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>14</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>8</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Patient propagules: Do soil archives preserve the legacy of fungal and prokaryotic communities?</ArticleTitle><Pagination><MedlinePgn>e0237368</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0237368</ELocationID><Abstract><AbstractText>Soil archives are an important resource in agronomic and ecosystem sciences. If microbial communities could be reconstructed from archived soil DNA, as prehistoric plant communities are reconstructed via pollen data, soil archive resources would assume even greater value for reconstructing land-use history, forensic science, and biosphere modelling. Yet, the effects of long-term soil archival on the preservation of microbial DNA is still largely unknown. To address this, we assessed the capacity of high-throughput sequencing (Illumina MiSeq) of ITS (internal transcribed spacer) and prokaryotic 16S rRNA genes for reconstructing soil microbial communities across a 20 years time-series. We studied air-dried soil archives and fresh soil samples taken from Populus bioenergy and deciduous forest research plots at the Kellogg Biological Station. Habitat and archival time explained significant amounts of variation in soil microbial α- and β-diversity both in fungal and prokaryotic communities. We found that microbial richness, diversity, and abundance generally decreased with storage time, but varied between habitat and taxonomic groups. The high relative abundance of ectomycorrhizal species including Hebeloma and Cortinarius detected in older soil archives raises questions regarding traits such as long-term persistence and viability of ectomycorrhizal propagules in soils, with relevance to forest health and ecosystem succession. Talaromyces, Paecilomyces and Epicoccum spp. were detected in fresh and across 20-year-old archived soils and were also cultured from these soils demonstrating their long-term spore viability. In summary, we found that microbial DNA in air-dried soils archived over the past 20 years degraded with time, in a manner that differed between soil types and phylogenetic groups of microbes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Benucci</LastName><ForeName>Gian Maria Niccolò</ForeName><Initials>GMN</Initials><Identifier Source="ORCID">0000-0003-1589-947X</Identifier><AffiliationInfo><Affiliation>Plant, Soil and Microbial Science Department, Michigan State University, East Lansing, MI, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rennick</LastName><ForeName>Bryan</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0002-0742-2279</Identifier><AffiliationInfo><Affiliation>Plant, Soil and Microbial Science Department, Michigan State University, East Lansing, MI, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonito</LastName><ForeName>Gregory</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Plant, Soil and Microbial Science Department, Michigan State University, East Lansing, MI, United States of America.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="N">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017753" MajorTopicYN="N">Ecosystem</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011387" MajorTopicYN="N">Prokaryotic Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017421" MajorTopicYN="N">Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList><CoiStatement>The authors have declared that no competing interests exist.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32780777</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0237368</ArticleId><ArticleId IdType="pii">PONE-D-20-15299</ArticleId><ArticleId IdType="pmc">PMC7418970</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>ISME J. 2019 Dec;13(12):3126-3130</Citation><ArticleIdList><ArticleId IdType="pubmed">31388130</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiome. 2017 Mar 3;5(1):27</Citation><ArticleIdList><ArticleId IdType="pubmed">28253908</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2006 Jun;9(6):683-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16706913</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 1993 Apr;2(2):113-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8180733</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22</Citation><ArticleIdList><ArticleId IdType="pubmed">20534432</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2013 Jul;199(1):288-99</Citation><ArticleIdList><ArticleId IdType="pubmed">23534863</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2017 Mar 24;12(3):e0173901</Citation><ArticleIdList><ArticleId IdType="pubmed">28339464</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2015 Nov 1;31(21):3476-82</Citation><ArticleIdList><ArticleId IdType="pubmed">26139637</ArticleId></ArticleIdList></Reference><Reference><Citation>PeerJ. 2019 Sep 26;7:e7744</Citation><ArticleIdList><ArticleId IdType="pubmed">31579614</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2019 Sep 1;366(17):</Citation><ArticleIdList><ArticleId IdType="pubmed">31603508</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Ecol. 2006 Sep;57(3):420-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16907756</ArticleId></ArticleIdList></Reference><Reference><Citation>Gigascience. 2012 Jul 12;1(1):7</Citation><ArticleIdList><ArticleId IdType="pubmed">23587224</ArticleId></ArticleIdList></Reference><Reference><Citation>Res Microbiol. 2009 Mar;160(2):89-98</Citation><ArticleIdList><ArticleId IdType="pubmed">19111612</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2006 Jul;72(7):5069-72</Citation><ArticleIdList><ArticleId IdType="pubmed">16820507</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2013 Oct;10(10):996-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23955772</ArticleId></ArticleIdList></Reference><Reference><Citation>Prikl Biokhim Mikrobiol. 2012 Jan-Feb;48(1):5-17</Citation><ArticleIdList><ArticleId IdType="pubmed">22567879</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2007 Aug;73(16):5261-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17586664</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2015 Mar;205(4):1525-36</Citation><ArticleIdList><ArticleId IdType="pubmed">25494880</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2010 Jun;307(1):80-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20412303</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Microbiol. 2016 Dec 19;2:16242</Citation><ArticleIdList><ArticleId IdType="pubmed">27991881</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2011 Jan;39(Database issue):D19-21</Citation><ArticleIdList><ArticleId IdType="pubmed">21062823</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol Rep. 2019 Apr;11(2):173-184</Citation><ArticleIdList><ArticleId IdType="pubmed">30507072</ArticleId></ArticleIdList></Reference><Reference><Citation>Elife. 2019 Sep 10;8:</Citation><ArticleIdList><ArticleId IdType="pubmed">31502536</ArticleId></ArticleIdList></Reference><Reference><Citation>J Microbiol Methods. 2010 Apr;81(1):48-55</Citation><ArticleIdList><ArticleId IdType="pubmed">20138194</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2004 Oct 29;306(5697):813</Citation><ArticleIdList><ArticleId IdType="pubmed">15514139</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014 Jan 28;9(1):e87217</Citation><ArticleIdList><ArticleId IdType="pubmed">24489873</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2014 Mar 1;30(5):614-20</Citation><ArticleIdList><ArticleId IdType="pubmed">24142950</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2014 Dec;12(12):789-90</Citation><ArticleIdList><ArticleId IdType="pubmed">25564681</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Comput Biol. 2014 Apr 03;10(4):e1003531</Citation><ArticleIdList><ArticleId IdType="pubmed">24699258</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2009 Jan;181(2):463-470</Citation><ArticleIdList><ArticleId IdType="pubmed">19121040</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2010 May;7(5):335-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20383131</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Jul 31;8(7):e70460</Citation><ArticleIdList><ArticleId IdType="pubmed">23936206</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2015 Mar;13(3):1</Citation><ArticleIdList><ArticleId IdType="pubmed">25685915</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2013 Nov;22(21):5271-7</Citation><ArticleIdList><ArticleId IdType="pubmed">24112409</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2017 Dec 06;18(1):538</Citation><ArticleIdList><ArticleId IdType="pubmed">29212440</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5881-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20231463</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2017 Jun;26(11):2895-2904</Citation><ArticleIdList><ArticleId IdType="pubmed">28261928</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Biol Sci. 2001 Dec 7;268(1484):2479-84</Citation><ArticleIdList><ArticleId IdType="pubmed">11747567</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><country name="États-Unis"><region name="Michigan"><name sortKey="Benucci, Gian Maria Niccol" sort="Benucci, Gian Maria Niccol" uniqKey="Benucci G" first="Gian Maria Niccol" last="Benucci">Gian Maria Niccol Benucci</name></region><name sortKey="Benucci, Gian Maria Niccol" sort="Benucci, Gian Maria Niccol" uniqKey="Benucci G" first="Gian Maria Niccol" last="Benucci">Gian Maria Niccol Benucci</name><name sortKey="Bonito, Gregory" sort="Bonito, Gregory" uniqKey="Bonito G" first="Gregory" last="Bonito">Gregory Bonito</name><name sortKey="Bonito, Gregory" sort="Bonito, Gregory" uniqKey="Bonito G" first="Gregory" last="Bonito">Gregory Bonito</name><name sortKey="Rennick, Bryan" sort="Rennick, Bryan" uniqKey="Rennick B" first="Bryan" last="Rennick">Bryan Rennick</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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